{"product_id":"silica-gel-in-silidispensetm-bottle-300-400-mesh-for-column-chromatography-400g-400","title":"Silica Gel in SiliDispense(TM) Bottle 300-400 mesh for Column Chromatography (400g) 400","description":"\u003ch3\u003eSilica Gel in SiliDispense(TM) Bottle 300-400 mesh for Column Chromatography (400g)\u003c\/h3\u003e\n\u003cp id=\"b\"\u003eSilica gel for column chromatography, 300-400 mesh medium particle size. SiO2\n300-400 mesh (75-38µm particle size), pH 6-7, pore size 60Å\n---------------------------------------- Lot Experimental Data Density\n(loose): 0.38g\/cm³ Density (well-packed): 0.45g\/cm³ Column volume: 42mL\/20g\nNormalized flow rate (gravity): 4.5mL\/min Normalized flow rate (2psi):\n31mL\/min While this silica will enable a fast flow, there is no need for a\nlong column. A short, thick column will generally be better, as long as sample\nis loaded as a thin layer and column is homogenously packed. Because if a TLC\nplate of 6-7cm long can separate your compound, so should and can a column of\n~10ish cm. A long column will only cause unnecessary peak broadening and if\nusing fine-size silica, high back-pressure. We care for you. No one should be\nexposed to silica dust. What causes the most harm may not be the most toxic,\nbut the most common reagent due to exposure amounts. With our specially\ndesigned and packed SilicaDispense(TM) bottles, say goodbye to the hassle and\nhazards of handling silica powder in your lab work. Easily twist open to uncap\nand squeeze out the silica directly into your column or final apparatus. You\ndo not need to transfer from a large container to a secondary container. You\ndo not need any tools or spatulas, while the SiliDispense(TM) bottles will fit\nin most lab spatulas if for any reason a spatula is to be used. Bottle size\ndesigned for benchtop storage and direct use. The sturdy, empty bottles can be\nreused for solvent storage and dispensing indefinitely. See photos and video\ndemonstration for this great convenience and safe health feature.\n---------------------------------------- The following is an actual separation\nexperiment performed with this silica: 20g of silica (300-400mesh) was added\nto a cotton-blocked glass chromatography column with I.D. 2.6cm (silica height\n~10cm). Silica column was then wetted and packed using AAdvance’s in situ wet\npack method. Column volume (lot) was 40mL. 0.2g dimethyl maleate (d.m.m.) and\n0.2g dimethyl fumerate (d.m.f.) were loaded onto column as a suspension in 5%\nethyl acetate in hexanes (4mL). Then, 10% ethyl acetate in hexanes was added\nas eluting solvent to start separation. Elution was conducted with a gravity\nflow and no extra pressure was applied. Solvent was collected with test tubes\nwith volumetric marks. The first fraction (d.m.f.) started eluting out at 70mL\nand finished eluting by 105mL. The second fraction (d.m.m.) started coming out\nat 125mL, and at the same time eluting solvent was replenished with 25% ethyl\nacetate in hexanes instead. Eluting finished at around 160mL. See separation\nresults by TLC spotting. This is a challenging separation, not only because of\nthe compounds’ closeness in Rf and polarity, but also due to the fact that\nd.m.f. dissolves poorly in hexanes or 5% ethyl acetate in hexanes, causing\npeak broadening.\u003c\/p\u003e \n\n\u003ch3\u003eProduct Features\u003c\/h3\u003e\n  \u003cul class=\"a\"\u003e\n         \u003cli\u003eSilica gel for column chromatography, 300-400 mesh medium particle size. SiO2 300-400 mesh (50-38m particle size), pH 6-7, pore size 60A\u003c\/li\u003e\n         \u003cli\u003eWhile this silica will enable a fast flow, there is no need for a long column. A short, thick column will generally be better, as long as sample is loaded as a thin layer and column is homogenously packed.\u003c\/li\u003e\n         \u003cli\u003eWith our specially designed and packed SilicaDispense(TM) bottles, say goodbye to the hassle and hazards of handling silica powder in your lab work.\u003c\/li\u003e\n         \u003cli\u003eYou do not need to transfer from a large container to a secondary container. Easily twist open to uncap and squeeze out the silica directly into your column or final apparatus.\u003c\/li\u003e\n         \u003cli\u003eYou do not need any tools or spatulas, while the SiliDispense(TM) bottles will fit in most lab spatulas if for any reason a spatula is to be used. Bottle size designed for benchtop storage and direct use.\u003c\/li\u003e\n         \u003cli\u003eAlso available in 200-300 mesh, 500-800 mesh. Please visit our storefront for more information.\u003c\/li\u003e\n  \u003c\/ul\u003e","brand":"AAdvance Instruments","offers":[{"title":"400","offer_id":48067930751127,"sku":"B0CVN14JLY","price":181.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0461\/8380\/9175\/files\/B0CVN14JLY.jpg?v=1785056228","url":"https:\/\/newnestshop.com\/products\/silica-gel-in-silidispensetm-bottle-300-400-mesh-for-column-chromatography-400g-400","provider":"NewNest Australia","version":"1.0","type":"link"}